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yeast expression vector ppiczab  (Thermo Fisher)


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  • 90

    Structured Review

    Thermo Fisher yeast expression vector ppiczab
    Yeast Expression Vector Ppiczab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+expression+vector+ppiczab/pm22922817-85-28-32
    Average 90 stars, based on 1 article reviews
    yeast expression vector ppiczab - by Bioz Stars, 2026-09
    90/100 stars

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    Amplification:

    Article Title: Overall neutralization of complement factor H by autoantibodies in the acute phase of the autoimmune form of atypical hemolytic uremic syndrome.
    Article Snippet: .. First, DNA encoding human FH domains 1–4 (residues 19–264) was amplified from a human liver cDNA library (Stratagene) with specific primers, and the product was inserted into the yeast expression vector pPicZaB (Invitrogen, Courtaboeuf, France). .. A His-tag was added to the C terminus of FH 1–4 using the QuickChange site-directed mutagenesis kit (Stratagene) with the following primer: 59-GGATGGCGTCCGTTGCCTTCATGTGAACATCATCATCATCATCATCATTGATCTAGAACA-39.

    cDNA Library Assay:

    Article Title: Overall neutralization of complement factor H by autoantibodies in the acute phase of the autoimmune form of atypical hemolytic uremic syndrome.
    Article Snippet: .. First, DNA encoding human FH domains 1–4 (residues 19–264) was amplified from a human liver cDNA library (Stratagene) with specific primers, and the product was inserted into the yeast expression vector pPicZaB (Invitrogen, Courtaboeuf, France). .. A His-tag was added to the C terminus of FH 1–4 using the QuickChange site-directed mutagenesis kit (Stratagene) with the following primer: 59-GGATGGCGTCCGTTGCCTTCATGTGAACATCATCATCATCATCATCATTGATCTAGAACA-39.

    Expressing:

    Article Title: Overall neutralization of complement factor H by autoantibodies in the acute phase of the autoimmune form of atypical hemolytic uremic syndrome.
    Article Snippet: .. First, DNA encoding human FH domains 1–4 (residues 19–264) was amplified from a human liver cDNA library (Stratagene) with specific primers, and the product was inserted into the yeast expression vector pPicZaB (Invitrogen, Courtaboeuf, France). .. A His-tag was added to the C terminus of FH 1–4 using the QuickChange site-directed mutagenesis kit (Stratagene) with the following primer: 59-GGATGGCGTCCGTTGCCTTCATGTGAACATCATCATCATCATCATCATTGATCTAGAACA-39.



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    90
    Thermo Fisher yeast expression vector ppiczab
    Yeast Expression Vector Ppiczab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yeast+expression+vector+ppiczab/pm22922817-85-28-32
    Average 90 stars, based on 1 article reviews
    yeast expression vector ppiczab - by Bioz Stars, 2026-09
    90/100 stars
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